Thrombocytosis induced in mice after subacute and subchronic V2O5 inhalation

Abstract

Reports about vanadium (V) inhalation toxicity on the hematopoietic system, specifically about coagulation are limited. Therefore, we decided to evaluate the effects of V with a complete blood count and morphologic analysis of platelets on blood smears. CD-1 male mice inhaled V₂O₅ 0.02 M 1 h twice weekly over 12 weeks. Blood samples were obtained by direct heart puncture; Wright stained smears were used for platelet quantification. An increase in platelet count from the third week of exposure was observed, as well as the presence of megaplatelets. Our results demonstrate, for the first time, that V induces thrombocytosis and it might correlate with some thromboembolic diseases. Further analysis is needed to evaluate the functionality of these platelets as well as the cause of its increase.

Keywords

inhalation exposure megaplatelets metals thrombocytosis vanadium References

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References

Avila-Costa, M. R. , Montiel-Flores, E. and Colin-Barenque, L. et al. 2004: Nigrostriatal modifications after vanadium (V2O5) inhalation. An immunocytochemical and cytological approach. Neurochemical Research 7, 1357-1362.

Beutler, E. , Lichtman, M. and Coller, B. et al. 2001: Williams hematology, sixth edition. McGraw-Hill, 2001.

Brauer, M. , Avila-Casado, M. C. and Fortoul, T. I. et al. 2001: Air pollution and retained particles in the lung. Environironmental Health Perspectives 109, 1039-1043.

Brook, R. D. , Franklin, B. and Cascio, W. et al. 2004: Air pollution and cardiovascular disease. A statement for healthcare professionals from the Expert Panel on Population and Prevention Science of the American Heart Association. Circulation 109, 2655-2671.

Clarke, R. W. , Coull, B. and Reinisch, U. et al. 2000: Inhaled concentrated ambiental particles are associated with hematologic and bronchoalveolar changes in canines. Environmental Health Perspectives 108, 1179-1188.

Dockery, D. W. and Pope, C. A. 1994: Acute respiratory effects of particulate air pollution. Annual Reviews of Public Health 15, 107-132.

Environmental Health Criteria 81 , 1988. Vanadium. International programme on chemical safety (IPCS). Retrieved from http://www.inchem.org/pages/ehc.html

Fortoul, T. I. , Saldivar, L. O. and Tovar, T. A. et al. 1996: Metals in lung tissue from autopsy cases in Mexico City residents. Comparison between cases from the 1950s and the 1980s. Environmental Health Perspectives 104, 630-632.

Fortoul, T. I. , Mendoza, M. L. and Avila, M. C. et al. 2002: Vanadium in ambient air. Concentrations in lung tissue from autopsies of Mexico City residents in the 1960s and 1990s. Autopsies from the sixties and the nineties. Archives of Environmental Health 57, 446-449.

10.

Gold, D. R. , Litonjua, A. and Schwartz, J. et al. 2000: The relationship between particulate pollution and heart rate variability. Circulation 101, 1267-1273.

11.

Ivancsits, S. , Pilger, A. and Diem, E. et al. 2002: Vanadate induces DNA strand break in cultured human fibroblasts at doses relevant occupational exposure. Genetic Toxicology and Environmental Mutagenesis 519, 25-35.

12.

Liao, D., Duan, Y. and Whitsel, E.A. et al. 2004: Association of higher levels of ambient criteria pollutants with