Levels of retinyl palmitate and retinol in the stratum corneum,epidermis,and dermis of female SKH-1 mice topically treated with retinyl palmitate

Abstract

Retinyl esters are the storage form of vitamin A in skin, and retinyl palmitate (RP) accounts for the majority of the retinyl esters endogenously formed in skin. RP is also obtained exogenously through the topical application of cosmetic and skin care products that contain RP. There is limited information on the penetration and distribution of RP and vitamin Awithin the stratified layers of the skin. The purpose of these studies was to determine the time course for accumulation and disappearance of RP and retinol in the stratified layers of skin from female SKH-1 mice that received single or repeated topical applications of creams containing 0.5 or 2% of RP. We developed an HPLC method with detection limits of 5.94 and 1.62 ng, to simultaneously quantify the amount of RP and retinol, respectively, in skin samples. Our results showed that RP rapidly diffuses into the stratum corneum and epidermal skin layers within 24 h following the application of RP-containing creams. Of the three skin layers, the highest level of RP and retinol per weight unit (ng/mg) at all time points was found in the epidermis. Levels of RP and retinol were lowest in the dermal layer and intermediate in the stratum corneum. The levels of RP and retinol in the separated skin layers and in the intact skin decreased with time, but levels of RP remained higher than control values for a period of up to 18 days. Our results indicate that the application of RP to mouse skin alters the normal physiological levels of RP and retinol in the skin.

Keywords

Mouse skin retinol retinyl palmitate

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References

Ahmad, H. and Mukhtar, H. 2004: Toxicology of the skin: new and emerging concepts. Toxicology and Applied Pharmacology 195, 265-266.

CTFA 1999: The Cosmetic, Toiletry and Fragrance Association. In Wenninger, J. , Canterbery, R. C. and McEwen, G. N. Jr. , editors, International cosmetic ingredient dictionary and handbook, eighth edition, 2000, 1279-1279.

FDA 2000: The US Food and Drug Administration. Data from FDA’s Voluntary cosmetics registration program.

IARC 1998: International Agency for Research on Cancer. Vitamin A; Volume 3, Handbooks of cancer prevention. French.

Idson, B. 1990: Vitamins in cosmetics, an update I. Overview and vitamin A. Drug and Cosmetic Industry 146, 26-91.

Kang, S. E. , Duell, A. and Fisher, G. J. , et al. , 1995: Application of retinol to human skin in vivo induces epidermal hyperplasia and cellular retinoid binding proteins characteristic of retinoic acid but without measureable retinoic acid levels or irritation. The Journal of Investigative Dermatology 105, 549-556.

Kurlandsky, S. B. , Duell, E. A. , Kang, S. , et al. , 1996: Auto-regulation of retinoic acid biosynthesis through regulation of retinol esterification in human kertinocytes. The Journal of Biological Chemistry 271(26): 15346-15352.

Mukhtar, H. 1995: Skin cancer: mechanisms and human relevance. CRC Press.

Ries, G. and Hess, R. 1999: Retinol: safety considerations for its use in cosmetics products. Journal of Toxicology Cutaneous and Ocular Toxicology 18, 169-185.

10.

Sklan, D. 1987: Vitamin A in human nutrition. Progress in Food and Nutrition Science 11, 39-55.

11.

Sorg, O. , Tran, C. and Carraux, P. , et al. , 1999: Retinol and retinyl ester epidermal pools are not identically sensitive to UVB irradiation and anti-oxidant protective effect. Dermatology 199, 302-307.

12.

Tavakkol, A. , Zouboulis, C. C. Duell, E. A. , et al. , 1994: A retinoic acid-inducible skin-specific gene (RIS-1/psoriasin): molecular cloning and analysis of gene expression in human skin in vivo and cultured skin cells in vitro. Molecular Biology Reports 20, 75-83.

13.

Tee, E. S. 1992: Carotenoids and retinoids in human nutrition. Critical Reviews in Food Science and Nutrition 31, 103-163.

14.

Torma, H. , Brunnberg, L. and Vahlquist, A. 1987: Age-related variations in acyl-CoA:retinol acyltransferase activity and vitamin A concentration in the liver and epidermis of hairless mice. Biochimica et Biophysica Acta 921(2), 254-258.

15.

Varani, J. , Warner, R. L. and Gharaee-Kermani, M. , et al. , 2000: Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin. The Journal of Investigative Dermatology 114(3), 480-486.

16.

Yan, J. , Xia, Q. and Webb, P. , et al. , 2006: Levels of retinyl palmitate and retinol in stratum corneum, epidermis, and dermis of female SKH-1 mice. Toxicology and Industrial Health 22, 103-112.