Melatonin modulates 900 MHz microwave-induced lipid peroxidation changes in rat brain

Abstract

Microwaves (MW) from cellular phones may affect biological systems by increasing free radicals, which may enhance lipid peroxidation levels of the brain, thus leading to oxidative damage. Melatonin is synthesized in and secreted by the pineal gland at night and exhibits anti-oxidant properties. Several studies suggest that supplementation with anti-oxidant can influence MW-induced brain damage. The present study was designed to determine the effects of MW on the brain lipid peroxidation system, and the possible protective effects of melatonin on brain degeneration induced by MW.

Twenty-eight Sprague-Dawley male rats were randomly divided into three groups as follows: (1) sham-operated control group (N-8); (2) study 900-MHz MW-exposed group (N-8); and (3) 900-MHz MW-exposed-melatonin (100 mg/kg sc before daily MW exposure treated group) (N-10). Cortex brain and hippocampus tissues were removed to study the levels of lipid peroxidation as malonyl dialdehyde. The levels of lipid peroxidation in the brain cortex and hippocampus increased in the MW group compared with the control group, although the levels in the hippocampus were decreased by MW-melatonin administration. The brain cortex lipid peroxidation levels were unaffected by melatonin treatment.

We conclude that melatonin may prevent MW-induced oxidative changes in the hippocampus by strengthening the anti-oxidant defense system, by reducing oxidative stress products.

Keywords

brain melatonin microwave oxidative stress

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References

Baydaş, G. , Kutlu, S. and Naz1rog'lu, M. et al. 2003: Inhibitory effects of melatonin on lipid peroxidation induced by intracerebroventricularly administered homocysteine. Journal of Pineal Research 34, 36-39.

Cabrera, J. , Reiter, R. and Tan, D. X. et al. 2000: Melatonin reduces oxidative neurotoxicity due to quinolinic acid: in vitro and in vivo findings. Neuropharmacology 39, 507-514.

Frey, A. H. 1998: Headaches from cellular telephones: are they real and what are the implications? Environmental Health Perspectives 106, 101-103.

Halliwell, B. and Gutteridge, J. M. C. 1999: Free radicals, other reactive species and disease. In Halliwell, B. and Gutteridge, J. M. C. , editors, Free radicals in biology and medicine, third edition. Oxford University Press, 639-645.

Ilhan, A. , Gurel, A. and Armutcu, F. et al. 2004: Ginkgo biloba prevents mobile phone-induced oxidative stress in rat brain. Clinica Chimica Acta 340, 153-162.

Irmak, M. K. , Fadillioglu, E. and Gulec, M. et al. 2002: Effects of electromagnetic radiation from a cellular telephone on the oxidant and antioxidant levels in rabbits. Cell Biochemistry and Function 20, 279-283.

Koyu, A. , Naz1rog'lu, M. and Ozguner, F. et al. 2005: Caffeic acid phenethyl ester modulates 1800 MHz microwave-induced oxidative stress in rat liver. Electromagnetics in Biology and Medicine 24, 135-142.

Kükner, A.Ş. , Kükner, A. and Naz1rog'lu, M. et al. 2004: Protective effects of intraperitoneal vitamin C, aprotinin and melatonin administration on retinal edema during experimental uveitis in the guinea pig. Cell Biochemistry and Function 22, 299-305.

Maharaj, D. S. , Maharaj, H. , Daya, S. and Glass, B. D. 2006: Melatonin and 6-hydroxymelatonin protect against iron-induced neurotoxicity. Journal of Neurochemistry 96, 78-81.

10.

McCann, J. , Dietrich, F. , Rafferty, C. and Martin, A. O. 1993: A critical review of the genotoxic potential of electric and magnetic fields. Mutation Research 297, 61-95.

11.

Murphy, J. C. , Kaden, D. A. , Warren, J. and Sivak, A. 1993: International Commission for Protection against Environmental Mutagens and Carcinogens. Power frequency electric and magnetic fields: a review of genetic toxicology. Mutation Research 296, 221-240.

12.

Naz1rog'lu, M. 2006: Effects of physical exercise with a dietary vitamins C and E combination on oxidative stress in muscle, liver and brain of streptozotocin-induced diabetic pregnant rat. Vitamin E: new research. Nova Science Publishers, in press.

13.

Naz1rog'lu, M. and Butterworth, P. J. 2005: Protective effects of dietary vitamin C and E combination on the blood antioxidative defense mechanism in rats with streptozotocin induced diabetes. Canadian Journal of Applied Physiology 30, 172-185.

14.

Pablos, M. I. , Reiter, R. J. and Chuang, J. I. et al. 1997: Acutely administered melatonin reduces oxidative damage in lung and brain induced by hyperbaric oxygen. Journal of Applied Physiology 83, 354-358.

15.

Pieri, C. , Marra, M. and Moroni, F. et al. 1994: Melatonin: a peroxyl radical scavenger more effective than vitamin E. Life Science 55, 271-276.

16.

Placer, Z. A. , Cushman, L. and Johnson, B. C. 1966: Estimation of products of lipid peroxidation (malonyl dialdehyde) in biological fluids. Analytical Biochemistry 16: 359-364.

17.

Rapacholi, M. H. 2001: Health risks from the use of mobile phones. Toxicology Letter 31, 323-331.

18.

Reiter, R. J. , Tan, D. X. , Osuna, C. and Gitto, E. 2000: Actions of melatonin in the reduction of oxidative status. Journal of Biomedical Science 7, 444-458.

19.

Wagner, P. , Roschke, J. and Mann, K. et al. 1998: Human sleep under the influence of pulsed radiofrequency electromagnetic fields: a polysomnographic study using standardized conditions. Bioelectromagnetics 19, 199-202.

20.

Watanabe, Y. , Nakagawa, M. and Miyakoshi, Y. 1997: Enhancement of lipid peroxidation in the liver of mice exposed to magnetic fields. Industrial Health 35, 285-290.