Alloys of chromium have a long history of success in the surgical treatment of many orthopaedic defects. Nonetheless, prostheses loosening are commonly found around arthoplasties due to corrosion of metals. On this basis, it is hypothesized that chromium accumulation interferes with remodeling of bone. The present study aims to analyse the toxic effects of chromium on bone phosphatases in various regions of the bone in rats. Rats were treated with chromium intraperitoneally (0.5 mg/kg) in the form of potassium dichromate for 5 days. The accumulation of chromium is approximately 5.2-fold in the vertebrae, 8.9-fold in the femur and 8.7-fold in the calvaria, when compared to control. Chromium administration significantly reduced the activity of enzymes, eg, alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP). The study revealed a significant increase in the concentration of calcium, altered bone formation rate and bone morphology in the femur, vertebrae and calvaria. The interesting findings of the current study suggest altered bone turnover.
1 Flaherty EJ.Physiologically-based models for boneseeking elements . Toxicol Appl Pharmacol1991; 111: 299-312 .
2.
2 Krane SM , Schiller AL , Banalis E.Metabolic bone disease. In Degroot LJ , Besser M , Burge HG , Jams JL , Loriaux DC , Marshal JC , et al. eds. Endocrinology. WB Saunders Co. , 1989: 1151-1165.
3.
3 Heaney RP.Effects of calcium on skeletal development: bone loss and risk of fractures . Am J Med1991; 91: 23S-28S .
4.
4 Collin P , Nefussi JR , Wetter A , Nicolas U , Boylefevre ML , Fleishch , et al. Expression of collagen, osteocalcin and bone alkaline phosphatase in a mineralizing rat osteoblastic cell culture . Calc Tiss Int1991; 50: 175-183 .
5.
5 Delmas PD.Markers of bone formation and resorption. In Favus MJ ed. Primer on the metabolic bone disease and disorders of mineral metabolism, second edition. Raven Press , 1993: 108-112.
6.
6 Talwar HS , Reddi AH , Menczel J , Thomas WC , Meyer JL.Influence of aluminium on mineralization during matrix-induced bone development . Kidney Int1986; 29: 1038-1042 .
7.
7 Shukla R , Borrschein RL , Dietrich KN , Buncher CR , Berger O , Hammond PB , et al. Effects of fetal and infant lead exposure on growth in stature . Pediatrics1989; 84: 604-612 .
8.
8 Howie DW , Rogers SD , McGee MA , Haynes DR.Biologic effects of cobalt chrome in cell and animal models . Clin Orthopaed1996; 329: 5217-5232 .
9.
9 Morais S , Sousa JP , Fernandes MH , Carvalho GS.In vitro biomineralization by osteoblast-like cells. I. Retardation of tissue mineralization by metal salts . Biomaterials1998a; 19: 13-21 .
10.
10 Morais S , Sousa JP , Fernandes MH , Carvalho GS , de Bruijn JD , van Blittevswijk CA.Decreased consumption of Ca and P during in vitro biomineralization and biologically induced deposition of Ni and Cr in presence of stainless steel corrosion products . J Biomed Mater Res1998b; 42: 199-212 .
11.
11 Neale SD , Haynes DR , Howie DW , Murray DW , Athanasou NA.The effect of particle phagocytosis and metallic wear particles on osteoclast formation and bone resorption in vitro . J Arthoplast2000; 15: 654-662 .
12.
12 Doran A , Law FC , Allen MJ , Rushton N.Neoplastic transformation of cells by soluble but not particulate forms of metals used in orthopaedic implants . Biomaterials1998; 19: 751-759 .
13.
13 Hsin-Yi Lin , Bumgardner JD.In vitro biocorrosion of CoCr Mo implant alloy by macrophage cells . J Orthopaed Res2004; 22: 1231-1236 .
14.
14 Brien WW , Salvati EA , Betts F , Bullough P , Wright T , Rimnac C , et al. Metal levels in cemented total hip arthoplasty. A comparison of well fixed and loose implants . Clin Orthopaed Rel Res1992; 276: 66-74 .
15.
15 Williams DF ed. Biocompatibility of orthopedic implants, Volume 1. CRC Press, Inc. , 1987 (Chapter 1 of Biocompatibility).
16.
16 Harmand MF.In vitro study of biodegradation of aCoCr alloy using a human cell culture model . J Biomater Sci Polym Educ1995; 6: 809-814 .
17.
17 IARC . IARC monograph on the evaluation of carcinogenic risks to humans in chromium, nickel and welding . WHO, 1990; 49- 49-256 .
18.
18 Carter DE.Oxidation-reduction reactions of metal ions . Environ Health Perspect1995; 103: 17-19 .
19.
19 Bagchi D , Stohs S , Downs B , Bagchi M , Preuss H.Cytotoxicity and oxidative mechanisms of different forms of chromium . Toxicology2002; 180: 5-22 .
20.
20 Granchi D , Ciapetti G , Savarino L , Stea S , Filippini F , Sudanese A , et al. Expression of CD69 activation antigen on lymphocytes of patients with hip prosthesis . Biomaterials2002; 21: 2059-2065 .
21.
21 Witmer CM , Harris R.Chromium content of bone after oral and intraperitoneal (IP) administration of chromium (VI) to rats . Toxicologist1991; 11: 41-41 .
22.
22 Weber H.Long-term study of the distribution of soluble chromate-51 in the rat after a single intratracheal administration . J Toxicol Environ Health1983; 11: 749-764 .
23.
23 Kraintz L , Talmage RV.Distribution of radioactivity following intravenous administration of trivalent chromium 51 in the rat and rabbit . Proc Soc Exp Biol Med1952; 81: 490-492 .
24.
24 Hopkins LL Jr.Distribution in the rat of physiological amounts of injected Cr51(III) with time . Am J Physiol1965; 209: 731-735 .
25.
25 Flaherty EJ.A physiologically based model of chromium kinetics in the rat . Toxicol Appl Pharmacol1996; 138: 54-64 .
26.
26 MacKenize RD , Byerrum RU , Decker CF , Hoppert CA , Langham RF.Chronic toxicity studies. II. Hexavalent and trivalent chromium administered in drinking water to rats . AMA Arch Ind Health1958; 18: 232-250 .
27.
27 Kargacin B , Squibb KS , Cosentino S , Zhitkovich A , Costa M.Comparison of the uptake and distribution of chromate in rats and mice . Biol Trace Elem Res1993; 36: 307-318 .
28.
28 Andersch MA , Szecypinski AJ.Use of pnitrophenyl phosphate as the substrate for determination of serum acid phosphatase . Am J Clin Pathol1947; 17: 571-574 .
29.
29 Tenniswood M , Bird CE , Clark AF.Acid phosphatases: androgen dependent markers of rat prostate . Can J Biol Chem1976; 54: 350-357 .
30.
30 Varely H. Calcium , phosphorus and phosphatase. In Practical clinical biochemistry, fourth edition. ArnoldHeinemann , 1988: 433-434.
32 Andrew DE , Lenore S , Arnold EG.Chromium estimation. In Standard methods for the examination of water and wastewater, nineteenth edition. 1995: 3.58-3.58.
33.
33 Okazaki Y , Gotoh E , Manabe T , Kobayashi K . Comparison of metal concentrations in rat tibia tissues with various metallic implants . Biomaterials2004; 25: 5913-5920 .
34.
34 Merritt K , Fedele CD , Brown SS. In Doherty PJ ed. Biomaterialtissue interfaces. Elsevier Science Publishers BV , 1992: 47.
35.
35 Cohen MD , Kargacin B , Klein CB , Costa M.Mechanisms of chromium carcinogenicity and toxicity . Crit Rev Toxicol1993; 23: 255-281 .
36.
36 Elias Z , Schneider O , Aubry F , Daniere M , Poirot O.Sister chromatid exchanges in Chinese hamster V79 cells treated with the trivalent chromium compounds chromic chloride and chromic oxide . Carcinogenesis1983; 4: 605-611 .
37.
37 Merritt K , Brown SA.Release of hexavalent chromium from corrosion of stainless steel and cobalt-chromium alloys . J Biomed Mater Res1992; 29: 627-633 .
38.
38 Allen MJ , Myer BJ , Millet PJ , Rushton N.The effects of particulate cobalt, chromium and cobalt-chromium alloy on human osteoblast-like cells in vitro . J Bone Jnt Surg1997; 79B: 475-481 .
39.
39 Ning J , Grant MH.Chromium(VI)-induced cytotoxicity to osteoblast-derived cells . Toxicol In Vitro1999; 13: 879-887 .
40.
40 Lohmann CH , Schwartz Z , Koster G , Jahn U , Buchhorn GH , MacDougall MJ , et al. Phagocytosis of wear debris by osteoblasts affects differentiation and local factor production in a manner dependent on particle composition . Biomaterials2000; 21: 551-561 .
41.
41 McCarthy EF , Frassica FJ.Anatomy and physiology of bone. In Pathology of bone and joint disorders. WB Saunders , 1998: 25-25.
42.
42 Buchman AL , Neely NBS , Grossie B Jr , Truong LMD , Lykissa E , Ahn C.Organ heavy-metal accumulation during parenteral nutrition is associated with pathologic abnormalities in rats . Nutrition2001; 17: 600-601 .
