Terminalia chebula Gertn. (Combetraceae) is an important herbal drug in Ayurvedic pharmacopea. In the present study, a 95% ethanolic extract of T. chebula (fruit) (TC extract), which was chemically characterized on the basis of chebuloside II as a marker, was investigated for hepatoprotective activity against anti-tuberculosis (anti-TB) drug-induced toxicity. TC extract was found to prevent the hepatotoxicity caused by the administration of rifampicin (RIF), isoniazid (INH) and pyrazinamide (PZA) (in combination) in a sub-chronic mode (12 weeks). The hepatoprotective effect of TC extract could be attributed to its prominent anti-oxidative and membrane stabilizing activities. The changes in biochemical observations were supported by histological profile.
2 Chatterjee S , Pakrasi SC . The treatise on Indian medicinal plants, Volume 3. National Institute of Science Communication and Information Resources2000: 203–204.
3.
3 Tasaduq SA , Singh K , Sethi S , Sharma SC , Bedi KL , Singh J et al. Hepatoprotective and antioxidant profile of HP-1, a polyherbal phytomedicine . Hum Exp Toxicol2003; 22: 639–646 .
4.
4 Malekzadeh F , Ehsanifar H , Shahamat M , Levin M , Colwell RR . Antibacterial activity of black myrobalan (Terminalia chebula Retz) against Helicobacter pylori . Int J Antimicrob Agents2002; 18: 85–88 .
5.
5 Shin TY , Jeong HJ , Kim DK , Kim SH , Lee JK , Kum DK et al. Inhibitory action of water soluble fraction of Terminalia chebula on systemic and local anaphylaxis . J Ethnopharmacol2001; 74: 133–140 .
6.
6 Vonshak A , Barazani O , Sathiyamoorthy P , Shalev R , Vardy D , Golan-Goldhirsh A.Screening South Indian medicinal plants for antifungal activity against cutaneous pathogens . Phytother Res2003; 17: 1123–1125 .
7.
7 Cheng HY , Lin TC , Yu KH , Yang CM , Lin CC . Antioxidant and free radical scavenging activities of Terminalia chebula . Biol Pharm Bull2003; 26: 1331–1335 .
8.
8 Suguna L , Singh S , Sivakumar P , Sampath P , Chandrakasan G.Influence of Terminalia chebula on dermal wound healing in rats . Phytother Res2002; 16: 227–231 .
9.
9 Saleem A , Husheem M , Harkonen P , Pihlaja K.Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula Retz fruit . J Ethnopharmacol2002; 81: 327–336 .
10.
10 Sabu MC , Kuttan R.Anti-diabetic activity of medicinal plants and its relationship with their antioxidant property . J Ethnopharmacol2002; 81: 155–160 .
11.
11 Ahn MJ , Kim CY , Lee JS , Kim TG , Kim SH , Lee CK et al. Inhibition of HIV-1 integrase by galloyl glucoses from Terminalia chebula and flavonol glycoside gallates from Euphorbia pekinensis . Planta Med2000; 68: 457–459 .
12.
12 Kour S , Grover IS , Singh M , Kaur S.Antimutagenicity of hydrolyzable tannins from Terminalia chebula in Salmonella typhimurium . Mutat Res1998; 419: 169–179 .
13.
13 Tamhane MD , Thorat SP , Rege NN , Dahanukar SA . Effect of oral administration of Terminalia chebula on gastric emptying: an experimental study . J Postgrad Med1997; 43: 12–13 .
14.
14 Sohni YR , Bhatt RM . Activity of a crude extract formulation in experimental hepatic amoebiasis and in immunomodulation studies . J Ethnopharmacol1996; 54: 119–124 .
15.
15 Kundu AP , Mahato SB . Triterpenoids and their glycosides from Terminalia chebula . Phytochemistry1993; 32: 999–1002 .
16.
16 Reen RK , Karan M , Singh K , Karan V , Johri RK , Singh J.Screening of various Swertia species extracts in primary monolayer cultures of rat hepatocytes against carbon tetrachloride-and paracetamol-induced toxicity . J Ethnopharmacol2001; 75: 239–247 .
17.
17 Germano MP , Angelo VD , Sanogo R , Catania S , Alma R , De Pasquale R et al. Hepatoprotective and antibacterial effects of extracts from Trichilia emetica Vahl. (Meliaceae) . J Ethnopharmacol2005; 96: 227–232 .
18.
18 Tasduq SA , Kaiser P , Koul S , Bhat R , Johri RK . Biochemical manifestations of anti-tuberculosis drugs induced hepatotoxicity and the effect of silymarin . Hepatol Res2005; 31: 132–135 .
19.
19 Post R , Sen AK . Sodium and potassium stimulated ATPase. In Estabrook RW , Pullman ME eds. Methods Enzymology. Academic Press, 1967: 762–768.
20.
20 Minotti G , Aust SD . The requirement of iron (III) in the initiation of lipid peroxidation by iron (II) and hydrogen peroxide . J Biol Chem1987; 262: 1098–1104 .
21.
21 Lowry OH , Rosebrough NJ , Farr AL , Randall RJ . Protein measurement with Folin phenol reagent . J Biol Chem1951; 193: 265–275 .
22.
22 Wong WM , Wu PC , Yuen MF , Cheng CC , Yew WW , Wong PC et al. Anti-tuberculosis drug-related liver dysfunction in chronic hepatitis B infection . Hepatology2000; 31: 201–206 .
23.
23 Wang C-J , Wang J-M , Lin W-L , Chu C-Y et al. Protective effect of hibiscus anthocyanins against ter-butyl hydroperoxide induced hepatic toxicity in rats . Food Chem Toxicol2000; 38: 411–416 .
24.
24 Caro AA , Cederbaum AI . Oxidative stress, toxicology and pharmacology of CYP 2E1 . Ann Rev Pharmacol Toxicol2004; 44: 27–42 .
25.
25 Freel RW , Goldner AM . Sodium coupled-electrolyte transport across epithelial layer: emerging concepts and directions . Am J Physiol1981; 241: G451–G460 .
26.
26 Garza-Ocana A , Zanatta-Calderon N , Acosta D , Torres-Alanis O , Pineyro-Lopez A.Production of reactive oxygen species by toxin T-514 of genus Karwinskia in vitro . Toxicol In Vitro2003; 17: 19–25 .
27.
27 Micu AL , Miksys S , Sellers E , Koop DR , Tyndale RF . Rat hepatic CYP 2E1 is induced by very low nicotine doses: an investigation of induction, time course, dose response, and mechanism . J Pharm Exp Ther2003; 306: 941–947 .
28.
28 Morimoto M , Zern MA , Hagbjork AL , Ingelman-Sundberg M , French SW . Fish oil, alcohol and liver pathology. Role of cytochrome P450 2E1 . Proc Soc Exp Biol Med1994; 207: 197–205 .
29.
29 Mourelle M , Muriel P , Favari L , Franco T.Prevention of CCl4-induced liver cirrhosis by silymarin . Fund Clin Pharm1989; 3: 183–191 .
