An Improved Calibration Method for the Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resononance Analysis of 15 N-Metabolically-Labeled Proteome Digests Using a Mass Difference Approach
Restricted accessResearch articleFirst published online June, 2012
An Improved Calibration Method for the Matrix-Assisted Laser Desorption/Ionization-Fourier Transform Ion Cyclotron Resononance Analysis of 15 N-Metabolically-Labeled Proteome Digests Using a Mass Difference Approach
High mass measurement accuracy of peptides in enzymatic digests is critical for confident protein identification and characterization in proteomics research. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) can provide low or sub-ppm mass accuracy and ultrahigh resolving power. While for ESI-FT-ICR-MS, the mass accuracy is generally 1 ppm or better, with matrix-assisted laser desorption/ionization (MALDI)-FT-ICR-MS, the mass errors can vary from sub-ppm with internal calibration to over 100 ppm with conventional external calibration. A novel calibration method for 15N-metabolically labeled peptides from a batch digest of a proteome is described which corrects for space charge induced frequency shifts in FT-ICR spectra without using an internal calibrant. This strategy utilizes the information from the mass difference between the 14N/15N peptide peak pairs to correct for space charge induced mass shifts after data collection. A procedure for performing the mass correction has been written into a computer program and has been successfully applied to high-performance liquid chromatography-MALDI-FT-ICR-MS measurement of 15N-metabolic labeled proteomes. We have achieved an average measured mass error of 1.0 ppm and a standard deviation of 3.5 ppm for 900 peptides from 68 MALDI-FT-ICR mass spectra of the proteolytic digest of a proteome from Methanococcus maripaludis.
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