The interaction between copper ions and γ-L-glutamyl–L-cysteinyl–glycine [glutathione (GSH)] molecules may lead to the formation of the physiologically occurring Cu(I)–[GSH]2 and Cu(II)–GSSG complexes. Since glutathione depletion in neurons and aberrant copper metabolism have been implicated in several neurodegenerative disorders, we studied here the interaction of GSH with copper ions (Cu2+) by electrospray ionization ion trap mass spectrometry (ESI-IT-MS). Besides, we extended this approach to pH in excess of 10 by adding ethanolamine to the solution being investigated. As a result, the ESI-IT-MS spectra revealed novel aspects regarding the speciation of copper–glutathione complex.
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