The lateral lipid distribution in eye lenses of three human donors were studied by matrix-assisted laser desorption ionization imaging mass spectrometry using a high mass resolution. By using exact mass measurements this study shows the relationship between the aging process and the number of lipids detected as well as between aging and the abundance of products derived from sphingomyelins by hydrolysis. Variable lipid composition was also observed in the nuclear, barrier, or cortex regions of the lens samples. This is the first study that suggests the distribution of lysolipids as a potential biomarker panel for the aging of human lens tissue.
DeeleyJ.M.HankinJ.A.FriedrichM.G.MurphyR.C.TruscottR.J.W.MitchellT.W.BlanksbyS.J., “Sphingolipid distribution changes with age in the human lens”, J. Lipid Res.51, 2753 (2010). doi: https://dx-doi-org.web.bisu.edu.cn/10.1194/jlr.M007716
5.
DeeleyJ.M.MitchellT.W.WeiX.J.KorthJ.NealonJ.R.BlanksbyS.J.TruscottR.J.W., “Human lens lipids differ markedly from those of commonly used experimental animals”, Biochim. Biophys. Acta1781, 288 (2008). doi: https://dx-doi-org.web.bisu.edu.cn/10.1016/j.bbalip.2008.04.002
CastaingR.SlodzianG., “Optique corpusculaire – premiers essais de microanalyse par emission ionique secondaire”, C. R. Hebd. Acad. Sci.255, 1893 (1962).
10.
SpenglerB.HubertM.KaufmannR., “MALDI ion imaging and biological ion imaging with a new scanning UV-laser microprobe”, Proceedings of the 42nd Annual Conference on Mass Spectrometry and Allied Topics, Chicago, Illinois, May 29 to June 3, 1994.American Society for Mass Spectrometry, Santa Fe, p. 1041 (1994).
11.
CaprioliR. M., “Method and apparatus for imaging biological samples with MALDI MS”, US Patent 5808300 A 19980915 (1998).
12.
PolJ.VidovaV.HyotylainenT.VolnyM.NovakP.StrohalmM.KostiainenR.HavlicekV.WiedmerS.K.HolopainenJ.M., “Spatial distribution of glycerophospholipids in the ocular lens”, PloS ONE6, e19441 (2011). doi: https://dx-doi-org.web.bisu.edu.cn/10.1371/journal.pone.0019441
13.
VidovaV.PolJ.VolnyM.NovakP.HavlicekV.WiedmerS.K.HolopainenJ.M., “Visualizing spatial lipid distribution in porcine lens by MALDI imaging high-resolution mass spectrometry”, J. Lipid Res.51, 2295 (2010). doi: https://dx-doi-org.web.bisu.edu.cn/10.1194/jlr.M005488
14.
StrohalmM.KavanD.NovakP.VolnyM.HavlicekV., “mMass 3: A cross-platform software environment for precise analysis of mass spectrometric data”, Anal. Chem.82, 4648 (2010). doi: https://dx-doi-org.web.bisu.edu.cn/10.1021/ac100818g
15.
SengJ.A.EllisS.R.HughesJ.R.MaccaroneA.T.TruscottR.J.W.BlanksbyS.J.MitchellT.W., “Characterisation of sphingolipids in the human lens by thin layer chromatography-desorption electrospray ionisation mass spectrometry”, Biochim. Biophys. Acta1841, 1285 (2014). doi: https://dx-doi-org.web.bisu.edu.cn/10.1016/j.bbalip.2014.05.006
16.
DeeleyJ.M.ThomasM.C.TruscottR.J.W.MitchellT.W.BlanksbyS.J., “Identification of abundant alkyl ether glycerophospholipids in the human lens by tandem mass spectrometry techniques”, Anal. Chem.81, 1920 (2009). doi: https://dx-doi-org.web.bisu.edu.cn/10.1021/ac802395d
