Electron Ionization Mass Spectra and Their Reproducibility for Trialkylsilylated Derivatives of Organic Acids,Sugars and Alcohols

Abstract

Mass spectra of trialkylsilyl derivatives of fatty acids, dicarboxylic acids, hydroxyacids, oxoacids, sugars, amino acids and alcohols were obtained. Amino acids were analyzed as tert-butyldimethylsilyl derivatives; all other model compounds were analyzed as trimethylsilyl derivatives. Reproducibility of the electron ionization (EI) mass spectra for the derivatives obtained was discussed. It was shown that, for many investigated derivatives, composition of the respective mass spectra depended greatly on ion source contamination. The trimethylsilylated α-tocopherol mass spectrum composition was most significantly influenced by ion source contamination. This compound can be used to test ion source contamination.

Keywords

electron ionization gas chromatography/mass spectrometry fatty acids dicarboxylic acids hydroxyacids oxoacids sugars amino acids alcohols trimethylsilyl derivatives mass spectrum reproducibility

Get full access to this article

View all access options for this article.

References

Molnar-Perl

, “Role of Chromatography in the Analysis of Sugars, Carboxylic Acids and Amino Acids in Food”, J. Chromatogr. A 891, 1 (2000).

Chance

D.L.

Gerhardt

K.O.

Mawhinney

T.P.

, “Gas-Liquid Chromatography–Mass Spectrometry of Primary and Secondary Fatty Alcohols and Diols as their tert-Butyldimethylsilyl Derivatives”, J. Chromatogr. A 771, 191 (1997).

Tetsuo

Zhang

Matsumoto

, “Gas Chromatographic–Mass Spectrometric Analysis of Urinary Sugar and Sugar Alcohols During Pregnancy”, J. Chromatogr. B 731, 111 (1999).

Kim

K.-R.

Kim

J.-H.

Jeong

D.-H.

Paek

D.-J.

Liebich

H.M.

, “Gas Chromatographic Analysis of Urinary Organic Acids from Nonsmokers and Smokers”, J. Chromatogr. B 701, 1 (1997).

Gehrke

C.W.

Leimer

, “Trimethylsilylation of Amino Acids. Effect of Solvents on Derivatization Using Bis(trimethylsilyl)trifluoroacetamide”, J. Chromatogr. 53, 201 (1979).

Gehrke

C.W.

Nakamoto

Zumwalt

R.W.

, “Gas-Liquid Chromatography of Protein Amino Acid Trimethylsilyl Derivatives”, J. Chromatogr. 45, 24 (1969).

Iwase

Takeuchi

Murai

, “Gas Chromatography–Mass Spectrometry of Trimethylsilyl Derivatives of Amino Acids”, Chem. Pharm. Bull. 27, 1307 (1979).

MacKenzie

S.L.

Tenaschuk

Fortier

, “Analysis of Amino Acids by Gas-Liquid Chromatography as tert-Butyldimethylsilyl Derivatives”, J. Chromatogr. 387, 241 (1987).

Starke

Kleinpeter

Kamm

, “Separation, Identification, and Quantitation of Amino Acids in L-Lysine Fermentation Potato Juices by Gas Chromatography–Mass Spectrometry”, Fresen. J. Anal. Chem. 371, 380 (2001).

10.

Zenkevich

I.C.

Ioffe

B.V.

, Interpretation of organic compounds mass spectra. Khimia, Leningrad, Russia (1986) (in Russian).