A heated capillary tube combined with a radio-frequency-only quadrupole has been coupled with a home-made, high-resolution orthogonal-injection, time-of-flight mass spectrometer to improve ion transmission from the atmospheric pressure to the low-pressure regions. With an electrospray ion source, the performance of the interface on the intensity of spectra was investigated. For electrospray ionization, the ion intensity detected on the time-of-flight mass spectrometer was seen to increase three-fold compared with an orifice interface. It has been shown that the enhanced ion inlet designs can not only increase the ion translation efficiency, but also improve the detection limits of the mass spectrometer. Coupling atmospheric pressure matrix-assisted laser desorption/ionization with the improved interface resulted in an instrument detection limit as low as 2.5 fmol.
WileyW.C.McLarenI.H., “Time-of-flight mass spectrometer with improved resolution”, Mass Spectrometer26, 1150 (1955).
2.
WilmM.ShevchenkoA.HouthaeveT., “Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry”, Nature379, 466 (1996). doi: 10.1038/379466a0
3.
GuilhausM.SelbyD.MlynskiV., “Orthogonal Acceleration Time-of-Flight Mass Spectrometry”, Mass Spectrom. Rev.19, 65 (2000). doi: 10.1002/(SICI)1098-2787(2000)19:2<65::AID-MAS1>3.0.CO;2-E
4.
ShafferS.A.TolmachevA.PriorD.C.AndersonG.A.UdsethH.R.SmithR.D., “Characterization of an Improved Electrodynamic Ion Funnel Interface for Electrospray Ionization Mass Spectrometry”, Anal. Chem.71, 2957 (1999). doi: 10.1021/ac990346w
CechN.B.EnkeC.G., “Practical Implications of Some Recent Studies in Electrospray Ionization Fundamentals”, Mass Spectrom. Rev.20, 362 (2001). doi: 10.1002/mas.10008
7.
MannM.WongS.F.FennJ.B., in Ion Formation from Organic Solids, Ed by HedinA.SundqvistB.BenninghovenA., John Wiley & Sons, New York, USA, p. 139 (1990).
8.
HawkridgeA.M.ZhouL.LeeM.L.MuddimanD.C., “Analytical Performance of a Venturi Device Integrated into an Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometer for Analysis of Nucleic Acids”, Anal. Chem.76, 4118 (2004). doi: 10.1021/ac049677l
HenionJ.D.CoveyT.R.BruinsA.P., US Patent 4,861,988 (1989).
11.
CoveyT.R., US Patent 5,412,208 (1995).
12.
ZhouL.YueB.DeardenD.V.LeeE.D.RockwoodA.L.LeeM.L., “Incorporation of a Venturi Device in Electrospray Ionization”, Anal. Chem.75, 5978 (2003). doi: 10.1021/ac020786e
13.
HawkridgeA.M.ZhouL.LeeM.L.MuddimanD.C., “Analytical Performance of a Venturi Device Integrated into an Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometer for Analysis of Nucleic Acids”, Anal. Chem.76, 4118 (2004). doi: 10.1021/ac049677l
14.
ShafferS.A.PriorD.C.AndersonG.A.UdsethH.R.SmithR.D., “An Ion Funnel Interface for Improved Ion Focusing and Sensitivity Using Electrospray Ionization Mass Spectrometry”, Anal. Chem.70, 4111 (1998). doi: 10.1021/ac9802170
15.
BelovM.E.GorshkovM.V.UdsethH.R.AndersonG.A.SmithR.D., “Zeptomole-Sensitivity Electrospray Ionization-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry of Proteins”, Anal. Chem.72, 2271 (2000). doi: 10.1021/ac991360b
16.
BelovM.E.GorshkovM.V.UdsethH.R.AndersonG.A.TolmachevA.V.PriorD.C.HarkewiczR.SmithR.D., “Initial Implementation of an Electrodynamic Ion Funnel with Fourier Trans form Ion Cyclotron Resonance Mass Spectrometry”, J. Am. Soc. Mass Spectrom.11, 19 (2000). doi: 10.1016/S1044-0305(99)00121-X
17.
PriorD.C.PriceJ.BruceJ.E., US Patent 6,455,846 (2002).
18.
WuS.ZhangK.KaiserN.K.BruceJ.E.PriorD.C.AndersonG.A., “Incorporation of a Flared Inlet Capillary Tube on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer”, J. Am. Soc. Mass Spectrom.17, 772 (2006). doi: 10.1016/j.jasms.2006.02.011
19.
RobinsonE.W.WilliamsE.R., “Multidimensional Separations of Ubiquitin Conformers in the Gas Phase: Relating Ion Cross Sections to H/D Exchange Measurements”, J. Am. Soc. Mass Spectrom.16, 1427 (2005). doi: 10.1016/j.jasms.2005.04.007
20.
GlishG.L.DanellR.M., US Patent 6,703,611 (2004).
21.
ChowdhuryS.K.KattaV.ChaitB.T., “An Electrospray-ionization mass spectrometer with new features”, Rapid Commun. Mass Spectrom.4, 81 (1990). doi: 10.1002/rcm.1290040305
22.
DawsonP.H.FrenchJ.B.BuckleyJ.A.DouglasD.J.SimmonsD., “The Use of Triple Quadrupoles for Sequential Mass Spectrometry, 2—A Detailed Case Study”, Org. Mass Spectrom.17, 205 (1982). doi: 10.1002/oms.1210170504
23.
LaikoV.V.BurlingameA.L., US Patent 5965884 (1999).
LaikoV.BurlingameA., “Atmospheric Pressure Matrix Assisted Laser Desorption Ionization Mass Spectrometry”, Presented at the 47th ASMS Conference on Mass Spectrometry and Allied Topics, Dallas, TX, June 1999.
26.
DoroshenkoV.M.LaikoV.V.TaranenkoN.I.BerkoutV.D.LeeH.S., “Recent development. in atmospheric pressure MALDI mass spectrometry”, J. Mass Spectrom.221, 39 (2002). doi: 10.1016/S1387-3806(02)00893-X
27.
DodonovA.F.KozlovskiV.I.SoulimenkovI.V.RazinkovV.V.LobodaA.V.ZhouZ.WollnikH., “High-resolution electrospray ionization orthogonal-injection time-of-flight mass spectrometer”, Eur. J. Mass Spectrom.6, 481 (2000).
28.
LinB.SunnerJ., “Ion transport by viscous gas flow through capillaries”, J. Am. Soc. Mass Spectrom.5, 873 (1994). doi: 10.1016/1044-0305(94)87012-8
29.
JiangL.MoiniM., “Design and performance of a high resolution electrospray ion source for a magnetic sector mass spectrometer with a heated capillary inlet”, J. Am. Soc. Mass Spectrom.6, 1256 (1995). doi: 10.1016/1044-0305(95)00552-8
30.
LazarI.M.LeeE.D.RockwoodA.L., “General considerations for optimizing a capillary electrophoresis-electrospray ionization time-of-flight mass spectrometry system”, J. Chromatogr. A829, 279 (1998). doi: 10.1016/S0021-9673(98)00743-2
31.
LazarI.M.XinB.M.LeeM.L., “Design of a ToF MS as a detector for CE”, Anal. Chem.69, 3205 (1997). doi: 10.1021/ac9700282
32.
VormO.RoepstorffP.MannM., “Improved resolution and very high sensitivity in MALDI-ToF of matrix surfaces made by fast evaporation”, Anal. Chem.66, 3281 (1994). doi: 10.1021/ac00091a044
33.
SchneiserB.B.LockC.CoveyT.R., “AP and vacuum MALDI on a QqLIT instrument”, J. Am. Soc. Mass Spectrom.16, 176 (2005). doi: 10.1016/j.jasms.2004.10.004
