Remote Raman Microimaging Using an AOTF and a Spatially Coherent Microfiber Optical Probe

Abstract

A fiber-optic Raman microimaging probe is described that is suitable for acquiring high-spatial-resolution Raman images in sampling situations with no clear line of sight. A high-power near-infrared diode laser combined with an acousto-optic tunable filter and a spatially coherent optical fiber bundle allow fluorescence-free Raman images of remotely located samples to be acquired at distances up to several meters. The feasibility of this technique is demonstrated with Raman images of (1) a pellet containing a mixture of a highly scattering sample, bis-methylstyrylbenzene (BMSB), KCl, and graphite, and (2) a partially graphitized diamond. These images clearly show phase boundaries over an area of approximately 0.1 mm² with ∼4-μm resolution.

Keywords

Raman imaging Raman spectroscopy Raman microprobe Fiber-optic Raman AOTF Raman endoscopy

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References

Hirschfeld

, J. Opt. Soc. Am. 63, 476 (1973).

Delhaye

and Dhamelincourt

, J. Raman Spectrosc. 3, 33 (1975).

Dhamelincourt

Wallart

Leclercq

N'Guyen

A. T.

and Landon

D. O

, Anal. Chem. 51, 414A (1979).

Barry

and Mathies

, J. Cell Biol. 94, 479 (1982).

Gardiner

D. J.

Gorvin

A. C.

Gutteridge

Jackson

A. R. W.

and Raper

E. S

, Corros. Sci. 25, 1019 (1985).

Sharma

S. K.

Mao

H. K.

Bell

P. M.

and Xu

J.-A

, J. Raman Spectrosc. 16, 350 (1985).

Gardiner

D. J.

Littleton

C. J.

and Bowden

, Appl. Spectrosc. 42, 15 (1988).

Corset

Dhamelincourt

and Barbillat

, Chem. in Britain 25, 612 (1989).

Treado

P. J.

and Morris

M. D

, Appl. Spectrosc. Rev. 29, 1 (1994).

10.

Puppels

G. J.

Schut

T. C. Bakker

Sijtsema

N. M.

Grond

Maraboeuf

de Grauw

C. G.

Figdor

C. G.

and Greve

, J. Mol. Struct. 347, 477 (1995).

11.

Garton

Batchelder

D. N.

and Cheng

, Appl. Spectrosc. 47, 922 (1993).

12.

Pallister

D. M.

Govil

Morris

M. D.

and Colburn

W. S

, Appl. Spectrosc. 48, 1015 (1994).

13.

Treado

P. J.

Levin

I. W.

and Lewis

E. N

, Appl. Spectrosc. 46, 1211 (1992).

14.

Morris

H. R.

Hoyt

C. C.

and Treado

P. J

, Appl. Spectrosc. 48, 857 (1994).

15.

Morris

M. D.

, Proc. SPIE-Int. Soc. Opt. Eng. 2385, 80 (1995).

16.

Kurtz

Dwelle

and Katzka

, Rev. Sci. Instrum. 58, 1996 (1987).

17.

Treado

P. J.

Levin

I. W.

and Lewis

E. N

, Appl. Spectrosc. 48, 607 (1994).

18.

Treado

P. J.

Levin

I. W.

and Lewis

E. N

, Appl. Spectrosc. 46, 553 (1992).

19.

Barbillat

Dhamelincourt

Delhaye

and Da Silva

, J. Raman Spectrosc. 25, 3 (1994).

20.

Dao

N. Q.

Jouan

and Sqalli

P. P. O

, Analusis Magazine 19, M43 (1991).

21.

Stellman

C. M.

Booksh

K. S.

Riddic

J. E.

and Myrick

M. L

, “Description and Performance of a Highly Versatile, Low-Cost Fiber-Optic Confocal Raman Microscope”, submitted to Rev. of Sci. Instrum. (1995).

22.

McCreery

R. L.

Fleischmann

and Hendra

, Anal. Chem. 55, 146 (1983).

23.

Schwab

S. D.

and McCreery

R. L

, Anal. Chem. 56, 2199 (1984).

24.

Schwab

S. D.

McCreery

R. L.

and Gamble

F. T

, Anal. Chem. 58, 2486 (1986).

25.

Schwab

S. D.

and McCreery

R. L

, Appl. Spectrosc. 41, 126 (1987).

26.

Hendra

P. J.

Ellis

and Cutler

D. J

, J. Raman Spectrosc. 19, 413 (1988).

27.

Allred

C. D.

and McCreery

R. L

, Appl. Spectrosc. 44, 1229 (1990).

28.

Myrick

M. L.

and Angel

S. M

, Appl. Spectrosc. 44, 565 (1990).

29.

Myrick

M. L.

Angel

S. M.

and Desiderio

, Appl. Opt. 29, 1333 (1990).

30.

Chong

C. K.

Shen

Fong

Zhu

Yan

F.-X.

Brush

Mann

C. K.

and Vickers

T. J

, Vib. Spectrosc. 3, 35 (1992).

31.

Schoen

C. L.

Cooney

T. F.

Sharma

S. K.

and Carey

D. M

, Appl. Opt. 31, 7707 (1992).

32.

Cooney

T. F.

Schoen

C. L.

Sharma

S. K.

and Carey

D. M

, Appl. Spectrosc. 47, 1683 (1993).

33.

Sharma

S. K.

Schoen

C. L.

and Cooney

T. F

, Appl. Spectrosc. 47, 377 (1993).

34.

Frank

C. J.

McCreery

R. L.

and Redd

D. C. B

, Anal. Chem. 67, 777 (1995).

35.

Walt

D. R.

Bronk

K. S.

Pantano

and Michael

K. L

, SPIE Proc. 2388, 554 (1995).

36.

Bronk

K. S.

and Walt

D. R

, Anal. Chem. 66, 3519 (1994).

37.

Healey

B. G.

and Walt

D. R

, SPIE-Proc. 2388, 519 (1995).

38.

Healey

B. G.

and Walt

D. R

, SPIE-Proc. 2388, 568 (1995).