Effects of Vitamin B 6 on Beta-Aminoproprionitrile–Induced Palatal Cleft Formation in the Rat

Abstract

Objective

This study was conducted to evaluate the effects of β-aminoproprionitrile and vitamin B₆ on palatal clefting.

Method

In four groups of pregnant Sprague-Dawley rats, β-aminoproprionitrile (BAPN; 600 mg/kg b.w.) was given by gavage on embryonal day 15, 7 hours to induce palatal clefts. Vitamin B₆ (10 mg/kg b.w., IM) was given twice on embryonal day 14, 7 hours and on day 15, 7 hours. The possibility that the food's content of vitamin B₆ affected the results was also tested. Palatal cleft formation was divided Into four different grades, ranging from no cleft formation to total cleft formation.

Results/conclusion

It was found that BAPN induces cleft palate in rat fetuses and that this defect can be prevented both in number and severity by administration of vitamin B₆ before and simultaneously with BAPN.

Keywords

beta-aminoproprionitrile embryogenesis palatal cleft prevention rats teratology

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