Effect of Aging and Rotation on Human Amnion Cell Response to Polio and Sindbis Viruses. ∗

Summary

1) The cytopathic response of primary human amnion cell cultures to chick embryo adapted Type II poliovirus was absent in stationary cultures inoculated at 5–10 days of age. In stationary cultures inoculated when 30 or more days of age, complete destruction of cells followed. Multiplication of virus in young cultures occurred in the absence of recognizable cytopathic change. Cytopathic response of cultures of approximately 14 days was markedly enhanced by rolling after inoculation. The effect of rolling was not clearly apparent when other strains of poliovirus including attenuated strains were tested. 2) Frequently the cytopathic response of amnion cultures to Sindbis virus was reduced or absent in young as compared with old cultures, but response of young cultures was variable. No effect of rolling on cytopathic response could be demonstrated. Viral multiplication was greater in older cultures.