Abstract
Summary
Two nutritional variants (JA-1, JA-2) have been developed from Jensen sarcoma cells cultured in vitro. Cells of the parental Jensen sarcoma required asparagine for growth, but those of the variant strains grew readily in the absence of exogenous asparagine. This variation in nutritional requirement has been retained in JA-1 and JA-2 for 23 and 16 subculture generations in vitro and in 3 and 18 in vivo transplant generations, respectively. Thus, this change was stable and heritable under the conditions described. Some growth and histological characteristics of these variants were described.
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