Hyperlipemia and Hemolysis. II. Interaction of Sodium Oleate with Human Erythrocytes in Homologous Plasma

Summary and Conclusion

Normal human plasma binds exogenous sodium oleate firmly in vitro as indicated by inhibition of oleate hemolysis despite vigorous mechanical agitation. The oleate hemolysis inhibition can be reduced 30-fold by adding oleate directly to whole blood, rather than to plasma subsequently reconstituted as whole blood. Whenever oleate enters whole human blood in vitro, red cells and plasma compete for binding oleate; that fraction of oleate bound by plasma is rendered hemolytically inert whereas that fraction bound by erythrocyte constitutes the hemolytically active moiety.