Abstract
Summary
1) Human kidney cultures were prepared successfully from tissues obtained following surgical removal or at autopsy from individuals of all ages, when specimens were collected promptly. 2) Baby kidney cells were superior to adult both for culture growth and for isolation and propagation of enteroviruses. 3) Poliovirus types 1, 2 and 3 (virulent and attenuated strains) grew well in both adult and baby HK cultures. In contrast, Coxsackie A9, B 1-5 and all ECHO viruses tested (except types 7 and 10) induced distinct cellular degeneration only in baby HK cultures, while incomplete destruction with low virus titers were obtained in cultures derived from certain adult human kidneys. 4) The advantages of using baby HK cultures for isolation of certain ECHO viruses are discussed.
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