Zone Electrophoretic Studies of Proteins and Glycoproteins of Bovine Serum and Synovial Fluid

Summary

1) Zone electrophoresis of 13 bovine sera yielded a mean concentration of 3.37 ± .29 g (42%) albumin, 1.19 ± .10 g (15%) alpha globulin, 1.23 ± .15 g (15%) beta globulin and 2.28 ± .49 g (28%) gamma globulin/100 ml. Serum protein-bound hexose was 124 mg/100 ml or 1.53 ± .12% of the mean serum protein concentration of 8.08 g/100 ml. Zone electrophoretic separation showed albumin to contain 7 ± 4%, alpha globulins 51 ± 6%, beta globulins 23 ± 4% and gamma globulins 20 ± 5% of the protein-bound polysaccharide. 2) Twenty-one synovial fluids from the same animals had a mean concentration of .90 ± .27 g (50%) albumin, .24 ± .08 g (14%) alpha globulins, .29 ± .10 g (16%) beta globulins and .37 ± .11 g (21%) gamma globulin/100 ml. Synovial fluid protein-bound hexose was 36 ± 11 mg/100 ml or 1.98 ± .46% of the mean synovial fluid protein of 1.80 g/100 ml. Zone electrophoretic separation showed albumin to contain 10 ± 5%, alpha globulins 49 ± 8%, beta globulins 28 ± 5%, and gamma globulins 14 ± 4% of the protein-bound polysaccharide. 3) The synovial fluid had relatively more albumin (50%) and relatively less gamma globulin (21%) than serum (albumin 42%, gamma globulin 28%) and a higher hexose/protein (PR) ratio indicating preferential permeability of albumin and one or more glycoproteins. Alpha globulins were not separated into alpha 1 and alpha 2 zones.