Abstract
Summary
A detailed examination of the free estrone fraction of alumina column chromatograms of extracts of blood plasma has shown that when elution is performed with 0.25, 0.50 and then 1% ethanol in benzene instead of only using 1% ethanol in benzene, the free “estrone” fraction actually consisted of a substance slightly less polar than estrone. Free “estrone” is seldom found in plasma. Two of 15 samples contained 7.6 and 2.3 μg/ 100 ml plasma. The fraction which presumably contained estradiol-17β contained other material. The so-called estradiol-17β fraction averaged 6.5 μg/100 ml plasma.
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