Viral Growth and Oncolysis in Krebs 2 Cells as Affected by Substrate. ∗

Summary

With 3 strains of influenza virus formation of non-infectious cell-associated hemagglutinins in Krebs 2 ascites cells was much greater in a medium containing lactate and glutamine than when glucose was substituted for lactate. Reduction in tumor-producing capacity of cell suspensions by action of influenza virus was correlated with the effect of substrate on HA formation. A marked oncolytic effect was evident in lactate medium at 4 hours, or before the increase in HA. Although Newcastle disease virus was oncolytic at the same concentration as influenza, growth of NDV and its action on tumor-producing capacity were not affected by the substrate.