Abstract
Summary
1) Sprague-Dawley female rats, age 50 days, were given 10 mg H3-3-methyl-cholanthrene dissolved in sesame oil by gastric tube. 2) In the first experiment selected tissues, urine and feces were analyzed for radioactivity after 24 hours. Sixty to 90% of administered radioactivity was found in gastric and intestinal contents or in feces; 4.4% in urine. Less than 2% was recovered in the tissues analyzed. Results obtained by combustion technic for measurement of total radioactivity were compared with those obtained by toluene extraction. Data indicate that most radioactivity in fat and mammary tissue could be extracted with toluene, whereas negligible quantities could be obtained by this method for liver, kidney, brain, uterus and skeletal muscle. 3) In the second experiment, 24-hour samples of urine and feces were analyzed for total radioactivity for 24-25 days. Significant quantities of tritium-labeled material appeared in urine for 13-17 days and in feces for 7-11 days. Thereafter, fecal and urinary excretion of radioactivity occurred intermittently in trace amounts.
We wish to thank Miss Beverly Cockrell for technical assistance.
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