Abstract
Summary
A method is described for simultaneous isolation and identification of poliovirus in stool specimens. The principal feature of the method is partial purification of virus by differential centrifugation of stool extract. Virus so prepared is free of nonspecific cytotoxins and is also more concentrated than in the original specimen. Results of studies on 176 stool specimens have shown that the method is rapid, sensitive, and free of complicating non-specific reactions. Positive specimens are typed in most cases within 5 days. The method should also enable more rapid isolation and identification of other enteroviruses.
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