Abstract
Summary
The preparation and standardization of a SBB - celite slurry for lipoprotein pre-staining is described. Dye uptake is proportional to lipid concentration and may be used to estimate chylomicrons, Sf 10-400 and Sf 0-10 lipoproteins isolated by density gradient ultracentrifugation. Precipitation on celite by altering solvent polarity is an effective means for preparing highly dispersed SBB and other lipid soluble substances. A polar dye contaminant is removed from SBB by solvent precipitation.
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