Metabolism of Human Amnion Cell Cultures Infected with Poliovirus. II. Utilization of Glucose,Pyruvate and Ribose in Virus Infected Cells

Summary

1. Infected amnion cells take up more glucose and produce more lactic acid than uninfected controls. However, the lactic acid:glucose ratio is 0.9 in infected and 1.6 in control cells. This finding indicates that poliovirus infected amnion cells use glucose through different metabolic channels than uninfected cells. 2. Both infected and uninfected cultures take up ribose from the medium, the infected cells being more active. The ribose consumed cannot supply the proper carbohydrate source for virus formation. Infected, but not uninfected cells, synthesize ribose, if glucose is present in the medium. 3. Both infected and uninfected cells take up pyruvate but its uptake is diminished following infection. Pyruvate cannot replace glucose for virus synthesis.