Abstract
Summary
Rabbits were immunized with finely ground, pulverized bovine cortical bone. It was demonstrated that the antibodies in the sera of these rabbits reacted with the bovine serum and the bovine red cell antigens present in the bone; however, after absorbing from these rabbit sera the bovine serum and bovine red cell antibodies, hemagglutination titers of low order still persisted. Grabar immunoelectrophoresis confirmed the results obtained by the hemagglutination assay and indicated the presence of 2 distinct antigens in bovine cortical bone unrelated to its serum and red cell content.
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