Abstract
Summary
Incubation of rat cortical slices for 2 hours in a non-substrated phosphate saline medium at room temperature, produces an “activating” medium which is capable of stimulating the oxygen uptake of freshly cut cortical slices when subsequently determined in it. The nature of the activating material is unknown. The presence of glucose (11.5 mm/l) or glucose plus additional substrates (Krebs III medium(4)) in the incubation medium masks the stimulation. Neither an increase in the temperature of incubation to 37.34°C, possibly allowing more “activating” material to diffuse into the medium, nor the presence of calcium in the incubation medium which would partially inhibit the respiration of brain tissue was sufficient to allow a stimulation to be observed in the presence of added substrates.
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