Effect of Insulin on D-Xylose Uptake by the Intact Rat Diaphragm

Summary

Increased D-xylose penetration of the intact rat diaphragm has been demonstrated at insulin concentrations of 50 to 500 microunits per ml, amounts that are within the range of physiological levels. Increased penetrability to D-xylose by preparations containing trypsin and chymotrypsin was observed at relatively high levels of the enzymes but not at molar concentrations equivalent to those of insulin at levels of 125 and 500 microunits per ml. The rates of penetration were approximately linear, but the day-to-day variations of this procedure were too large for a useful bioassay method.