Demonstration of the Inherited Serum Group Specific Protein by Acrylamide Electrophoresis. ∗

Summary

Acrylamide gel electrophoresis in a vertical slab has been adapted to the routine analysis of the Gc protein. The band patterns do not differ in principle from those obtained by starch gel electrophoresis using a discontinuous buffer system; however, the Gc bands are very much sharper and the fast Gc components are well separated from the trailing edge of the albumin. The method has good reproducibility and may be capable of still further refinement. At present it provides a simple and convenient way of confirming the results obtained from immunoelectrophoresis and is particularly advantageous in investigation of rapidly migrating Gc variants which are only with considerable difficulty distinguished by starch gel electrophoresis.