Lipogenesis and Enzyme Activity in Rat and Mouse Epididymal Adipose Tissue

Summary

The ability of rat and mouse epididymal adipose tissue to incorporate acetate-1- 14 C into fatty acids was studied. The activity of glucose-6-phosphate dehydroge-nase (G-6-PD), 6-phosphogluconate dehy-drogenase (6-PGD), NADP malic dehy-drogenase (malic enzyme), NAD malic de-hydrogenase, glutamic-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) in these tissues was also determined. Mouse adipose tissue incorporated significantly more acetate-1-¹⁴C into fatty acids and exhibited higher G-6-PD, 6-PGD and malic enzyme activity than comparable tissue from rats. The malic dehydrogenase activity of rat and mouse adipose tissue was not different but was considerably higher than the activity of the other dehydrogenases studied. The activity of GPT was higher in rat adipose tissue, and that of GOT was higher in tissue from mice. The GPT/GOT ratio was higher in rat than in mouse adipose tissue (5.1 vs 1.7).