Enumeration of VSV Particles and a Demonstration of Their Growth Kinetics by Electron Microscopy. ∗

Summary

Growth curve studies indicate that the m.o.i. plays a major role in the replication and resultant quantity of VSV. Consistent high yields of the virus can be obtained when the m.o.i. is kept at approximately 1. Natural aggregation studies using the count technique indicate that the virus is extruded from the cells in loosely connected chains. The agar sedimentation procedure for counting virus particles can be readily adapted to vesicular stomatitis virus with high efficiency.

The author is most grateful for the excellent technical assistance of Mary-Martin Bratcher.