Abstract
Summary
In the present paper the anti-hemostatic effect of heparin on rat tail preparations was studied. It was confirmed that the antihemostatic effect of heparin is not removed after several washes with saline solutions and it was shown that its activity is closely related to the sulfate groups of the heparin molecule. The coagulation time of samples collected from oozing blood in the heparin-treated tail remained in the normal range.
Several salts, nucleotides and proteins were tested for ability to remove this antihemostatic effect. Of the substances tested, phosphate and sulfate ions, ATP, ADP and myosin were able to remove the antihemostatic activity while salts and proteins involved in blood coagulation were without effect. The effect of ATP could be shown to be accompanied by the release of heparin to the bath fluid by a microelectrophoresis technique.
The inhibition by heparin of myosin ATP-ase activity was demonstrated in vitro.
The role of muscular contraction, coagulation, and platelet adhesiveness on hemostasis is discussed.
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