Temperature Dependence of the Synthesis of Adenovirus Tumor and Viral Antigens ∗

Summary

The synthesis of adenovirus T and V antigens in infected cell cultures incubated at different temperatures was studied. At low temperature (24°C) development of V antigens was limited, while significantly larger amounts of T antigen were produced. Raising the incubation temperature from 24 to 37°C led to a rapid increase in V antigen. Highest titers of T antigen for adenovirus types 12, 18, and 31 were obtained in Vero and RK-1 cells incubated at 30°C. The antigen produced in the cultures was found to be unusually cross-reactive with type 7 tumor antibody. Thermal separation of the synthesis of adenovirus T and V antigens in cell cultures should provide a method for the production of adenovirus T an tigens.