Variable Results of Sodium Deoxycholate Tests of Nodamura Virus,an Ether and Chloroform Resistant Arbovirus ∗

Summary

Nodamura virus, an ether and chloroform resistant arbovirus, was likewise resistant to sodium deoxycholate by either of two SDC test methods when virus was tested in suspensions of infected suckling mouse brain, but by only one of two methods when tested in suspensions of infected suckling mouse muscle. By the method in which muscle suspensions of virus were incubated with 0.5% final concentration of SDC at 37° for 1 hr, and then diluted and tested in mice, inactivation occurred irregularly and unpredictably. These variable results were unexplained, but were not due to brand of SDC, duration of incubation, individual muscle suspensions, concentration of muscle tissue in virus-SDC mixtures, passage history of virus in mice or arthropods, dates of experiments or methods of sterilizing SDC. However, the results indicated that tissue source of virus and method of performing SDC tests can influence results.