Abstract
Summary
This paper describes a rapid and sensitive radio-immunoassay for glucagon, in which a powdered cellulose suspension is used for the separation of free from antibody-bound glucagon. One hundred samples can be assayed conveniently in 2 days. The method was applied to the assay of glucagon in serum and in incubation media. Isolated rat pancreatic islets incubated in Krebs—Henseleit buffer containing glucose at a concentration of 300 mg/100 ml secreted glucagon at an average rate of 42 ng/10 islets/hr. When the concentration of glucose was 30 mg/100 ml the average rate of glucagon secretion was 72 ng/10 islets/hr (p< 0.005).
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