Tumor Antigens II. Immunological Studies of Tissue and Tumor Antigens and Serum Protein Production by Murine Interstitial Cell Tumors of the Testis and a Hepatoma 1

Summary

Several murine ICT's and a murine hepatoma were studied for soluble antigens and differences in serum protein production in vitro by the tumors as compared to their normal counterparts. The technique used was that of radioimmunoelectrophoresis. A soluble antigen was not found in culture fluids obtained from the hepatoma but was present in two of the four ICT's. Its concentration in the normal testes was significantly less than that of the tumors. All of the ICT's synthesized B_1C but the labeling was not as prominent as that obtained with culture fluids obtained from the normal testes. Three of the four varieties also produced Tr. The labeling of Tr and B_1C by these tumors and tissues is believed to be due to the presence of infiltrating host cells. The hepatoma was found to synthesize albumin and B_1C.

The excellent technical assistance of Mrs. Yvonne Simmons is gratefully acknowledged.