Abstract
Summary
Three lines of evidence are presented which show that the mechanism whereby the addition of HCG confers FSH specificity on the FSH radioimmunoassay is by adsorbing out a species of antibodies which fails to distinguish among the glycoprotein hormones, FSH, LH, and HCG, leaving a second species of FSH-specific antibodies to react with the FSH label in the system.
The authors wish to acknowledge the technical assistance of Mrs. P. Schultz, Miss N. Highum, and Mrs. F. Smale. This work was supported by the Medical Research Council of Canada, Grant MA-2997 and the USPHS, Grant HD-3726.
Get full access to this article
View all access options for this article.