Propagation of Mammalian Viruses in Protista. IV. Experimental Infection of C. albicans and S. Cerevisiae with Polyoma Virus 1

Summary

Experimental infection of yeasts, C. albicans and S. cerevisiae with unadapted polyoma virus was carried out reproducibly under various experimental conditions, measured by increase in hemagglutination and infectivity during incubation. All important assays were repeated several times with similar positive results. Approximately 68-fold increase in HAU and approx 10⁴ increase in PFU/system over the inoculum was observed with unadapted PyV, but this yield may be increased with adaptation and further technical refinements as will be published separately. The virus propagation brought about cell biological and population changes which were illustrated and discussed together with the significance and bearing of the primary infection of two different species of yeasts with an oncogenic virus.

The courtesy of Dr. M. Williams of the Ontario Cancer Institute for the T strain of the PyV, Mr. G. Healy, Connaught Medical Research Laboratories, for the generous supply of media, Dr. D. B. W. Reid, Professor of Biometry, University of Toronto, for advice in statistical evaluation and Mr. J. Kovács for his technical help is gratefully acknowledged.