Effects of Ischemia on the Concentration of Adenine Nucleotides in the Kidney of Anesthetized Dogs 1

Summary

Ischemia causes a rapid decline in the concentration of ATP in the kidney cortex of anesthetized dogs. Within 8 sec of ischemia distribution of adenine nucleotides is determined by activity of adenylate kinase. Nucleotide metabolism of the cortex of dog is distinguished from that of rat by the observation that concentrations are lower and by the evidence of activity of adenylate kinase. We estimate that blood-flow dependent ATP turnover in the cortex is 27% of that calculated on the basis of renal O₂ consumption. The medulla, in contrast to the cortex, maintains its ATP concentration during 60 sec of interruption of blood flow. Studies of nucleotide metabolism in the kidney must include consideration of differences in adenylate kinase activity between cortex and medulla and between different mammalian species.