The Loss of Growth Vitality of Human Lymphoid Cell Lines Derived from Healthy Adults 1

Summary

Under the culture conditions described in this report, 20 lines of cells morphologically similar to the lymphoid cells were derived from the blood of eight healthy adults. Four of the 20 lines have undergone 85 to 107 serial divisions and are still growing vigorously. Fifteen lines showed an unexplained loss in growth vitality after the 3rd to 48th serial division. One line was contaminated by fungi in the 8th serial division.