Abstract
Summary
The indigogenic technique, a simple, specific, and reproducible method for demonstrating hydrolytic enzymes, has been adapted for electron microscopy, β-d-Galacto-sidase has been demonstrated in rabbit alveolar macrophages and other cell types with the same halogenated indoxyl substrate used in light microscopy. The reaction product is substantive and is readily identified in the electron microscope. The method is applicable to other hydrolytic enzymes.
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