Separation of Intrinsic Factor Antibodies from Parietal Cell Antibodies in Pernicious Anemia Serum by Gel Filtration 1

Summary

The separation of IFA from PCA in PA serum was achieved by coupling the IFA with the partially purified human IF and eluting this relatively large complex in the void volume on a Sephadex G-200 column. The dissociation of the IF-IFA complex was achieved, thereafter, by treatment with 0.1 N HCl and elution of the IFA in the void volume of the G-150 Sephadex column, while IF was retarded on this column.