Heparin-Induced Release of Lipase Activity From Perfused Canine Subcutaneous Adipose Tissue 1

Summary

The effects of heparin, histamine, and Compound 48/80 on the release of free fatty acids (FFA), glycerol, and venous plasma lipolytic activity (VPLA) from the isolated perfused canine subcutaneous adipose tissue in situ was studied. Intra-arterial administration of heparin with dosages between 5 to 500 μg produced an increase in VPLA release which was dose dependent. The VPLA release was decreased after repeated injection of heparin. Heparin administration did not alter the release of FFA and glycerol. Histamine and Compound 48/80 increased FFA and glycerol release, but produced no effect on VPLA release. The results do not support the contention concerning a possible role of mast cell heparin in the regulation of lipid metabolism in canine subcutaneous adipose tissue but confirm the previous findings that mast cell histamine may exert some effect in the mobilization of FFA.