Transformation of Fischer Rat Embryo Cells by the Combined Action of Murine Leukemia Virus and (-)- Trans -Δ 9 -Tetrahydrocannabinol 1

Summary

The cannabinoid, Δ⁹THC [(-)-trans-Δ⁹-tetrahydrocannabinol], was found to transform high passage Fischer rat cells chronically infected with Rauscher leukemia virus, but the transformation could be demonstrated only after extended subculture (13 passages) of the cells. Under the same conditions, control cultures and cultures treated with Δ⁸THC [(-)-trans-Δ⁸-tetrahydrocannabinol], CBN [cannabinol], or CBD [transcannabinol], were not transformed (20 passages). In contrast, cultures treated with 3MC [3-methylcholanthrene] were transformed in three subpassages. Thus, Δ⁹THC is the most active of the major cannabinoids as a transforming agent, but this activity is weak as compared to 3MC.