The Production of Migration Inhibitory Factor by Nonimmune Guinea Pig Lymphoid Cells Incubated with Concanavalin A 1

Summary

Migration inhibitory factor (MIF) is produced upon concanavalin A (Con A) stimulation of nonimmune lymphoid cells. This production is specific for the α-1, 4-arabinopyranoside affinity of the Con A as demonstrated by the inhibitory effect of methyl-α-D-mannoside. Con A-induced MIF synthesis is puromycin sensitive. Con A-induced MIF can be separated from Con A by column chromatography through Sephadex G-200. This mitogen-induced mediator seems to have approximately the same molecular weight as the antigen-induced MIF as measured by gel filtration. MIF can be produced by lymph node cells in the presence of serum supplemented media. Spleen cells do not require serum-supplemented media to produce Con A-induced MIF.