The Effect of Deconjugated and Conjugated Bile Salts on the Intestinal Uptake of Radio–vitamin B 12 in Vitro and in Vivo 1

Summary

Deconjugated cholic acid decreased the intestinal uptake of radio–B₁₂ by the guinea pig intestinal mucosal homogenate by 50 to 55%. It also decreased the absorption of radio–B₁₂ in 2 normal adult volunteers as measured by the double label hepatic uptake test. Cholic acid was felt to produce this decrease by a direct toxic effect on the ileal mucosa. Deoxycholic acid was found to produce a slight but not statistically significant decrease in the uptake of radio–B₁₂ by the guinea pig intestinal mucosal homogenate. Glycocholic acid, and glycodeoxycholic acid had no statistically significant effect on radio–B₁₂ uptake by the guinea pig intestinal mucosal homogenate.