In Vitro Binding of Mixed Micellar Solutions of Fatty Acids and Bile Salts by Cholestyramine

Summary

When cholestyramine was incubated with pure bile salts in isotonic saline, it preferentially bound taurine-conjugated bile salts and dihydroxy bile salts as compared with the respective glycine conjugates and trihydroxy bile salts. Addition of fatty acids to the incubation mixtures resulted in decreased binding of each bile salt, an effect directly related to concentration of fatty acid. Medium-chain fatty acids had relatively little effect on binding, whereas the longer chain fatty acids markedly depressed binding of each of four pure conjugated bile salts in isotonic saline and of naturally occurring mixed bile salts in swine bile.