Abstract
Summary
Dengue virus type 2 has been cloned in hamster kidney cells, a noncyto-pathic, noncytocidal virus-cell system, utilizing a direct fluorescent antibody method. A clone is considered to have arisen from the infection of a susceptible cell by a single infectious particle of DEN-2.
This work was performed under the sponsorship of the Commission on Viral Infections, Armed Forces Epidemiological Board, and was supported by the U. S. Army Medical Research and Development Command under Contract No. DADA17-69-C-9048 and by Public Health Service Research Grant No. AI-02686 from the National Institute of Allergy and Infectious Diseases. The application of direct fluorescent antibody methods to dengue viruses was achieved by Drs. Robert W. Atchison and J. Jayavasu, who also prepared the conjugated immune ascitic fluids. Most able technical assistance was provided by Alma Tillman, Annie McCoy, Carlie White, and Frances Brisset.
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