Characterization of Echovirus Types 7,12,and 29 Hemagglutinins for Human and Monkey Erythrocytes

Summary

Enterovirus field and prototype strains were tested for agglutination using human and African Green Monkey erythrocytes. Only ECHOvirus types 7, 12, and 29 agglutinated monkey erythrocytes; the titers were similar to those obtained with human erythrocytes. Passage of these ECHOviruses in human diploid fibroblast cells caused a reduction in hemagglutination titers of some of the strains. Human and monkey erythrocytes after prior adsorption and elution still retained their receptors for ECHOvirus hemagglutinins. Treatment of ECHOvirus 29 hemagglutinin with trypsin removed combining sites for receptors on monkey erythrocytes but not those on human erythrocytes. No differences in ECHOvirus types 7 and 12 hemagglutinins for human erythrocytes or monkey erythrocytes were detected by chemical treatment of these viruses.