Bromodeoxyuridine-Inhibition of Substratum-Controlled Chondrogenesis 1 2

Summary

Upon a substratum of insoluble bone matrix gelatin (BMG) in tissue culture, mesenchymal cells migrate out of muscle, proliferate, reaggregate on the second day and develop into cartilage on the eighth day. Mesenchymal cells proliferating in culture media containing BrdU during the first 2-3 days are irreversibly inhibited from differentiation and development of cartilage. In media containing BrdU during any interval between 3 and 8 days of culture, inhibition is reversible and cartilage develops almost invariably. Hence acquisition and stabilization of the differentiated state occurs in less than 3 days and within the 24 hr interval between 2 and 3 days coinciding with the time of interaction of mesenchymal cell filopods with surface substances of BMG.