Enhanced Establishment of Lymphoblastoid Cell Lines With the Ficoll-Hypaque Density Gradient Technique

Summary

Lymphoblastoid cell lines were established using peripheral blood which was obtained from ten patients with systemic lupus erythematosus, eight patients with infectious mononucleosis and 19 normal individuals. In each instance, the blood was divided into two portions. One portion was prepared for culture by gravity sedimentation of the white blood cells. The other portion was prepared by use of the Ficoll-Hypaque density gradient technique for separation of pure mononuclear cells. Results demonstrated that the Ficoll-Hypaque technique was significantly more efficient for establishment of lymphoid suspension cultures than was the gravity sedimentation method.