Abstract
Summary
Two FeLV fractions from Se-phadex G-150 gel filtration were used to sensitize RBC for the PHA test. When the cells were coated with the fraction from the second peak consisting mostly of a major gs antigen of FeLV, no antibodies could be detected either in hyperimmunized cat sera or sera from leukemic cats, whereas antibodies were readily detectable in immunized rabbits. Using cells coated with the first peak eluants or Tween-ether disrupted FeLV, PHA antibodies were detected in cat sera. There existed, however, one significant exception that a cat with the diagnosis of mast cell leukemia showed antibody against the second peak fraction. Little or no antibodies could be detected in cat sera by CF or gel-diffusion. There was some correlation between hemagglutinating antibodies and con-glutinating complement absorbing antibodies, but these antibodies did seem to differ from neutralizing antibody.
This research was supported by NIH Grant No. CA-13085 and in part by Contract No. NIH-NCI-71-2508 within the Virus Cancer Program of the National Cancer Institute. We thank Dr. J. E. Post for supplying sera from leukemic cats, and Dr. C. L. Gries for his help in the performance of cat experiments.
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